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histagged cd11b  (R&D Systems)


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    R&D Systems histagged cd11b
    (A) WT or <t>CD11b</t> −/− mice were challenged i.v. with 25,000 viable cells of WT or ece1 Δ/Δ C. albicans , and cerebral clearance was assessed over 20 days. (B–D) Schematic diagram of fungistasis assay (B) and aggregate data of candidalysin-activated BV-2 cells challenged with either WT or ece1 Δ/Δ C. albicans with and without anti-CD11b or NIF blockade of CD11b (C and D). (E and F) Fungistasis assay of candidalysin-activated (E) primary microglia or (F) primary astrocytes from WT mice with WT C. albicans with and without anti-CD11b or NIF blockade of CD11b. (G) Pull-down assays using CD11-expressing CHO cells. CHO-CD11a/b/c cell lysates (prey) were incubated with biotinylated candidalysin (Bio-Clys; bait) or biotinylated serine (Bio-serine; control), and the mixtures were loaded onto prewashed streptavidin beads. Bait-prey complexes were eluted from the beads and loaded onto SDS-PAGE gels to detect CD11a, CD11b, and CD11c via western blotting. (H–J) Schematic diagrams and aggregate data depicting in vitro assays in which the dose-dependent binding of plate-bound (H) CD11b extracellular domain or (I) candidalysin/SC peptide to the other reagent or (J) CD11b or CD11c extracellular domains binding to plate-bound candidalysin were determined colorimetrically (OD, optical density). (K) Flow cytometric analysis of splenocytes from WT or CD11b −/− mice incubated with AF647-conjugated CL (10 μM). n = 4, mean ± SEM, *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001 using one-way ANOVA followed by Tukey’s test for multiple comparison (A–F), two tailed Student’s t test (H–J), or two-way ANOVA (K). Data are representative of two or three independent experiments.
    Histagged Cd11b, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/histagged+cd11b/Recombinant+Mouse+Integrin+alpha+M+beta+2+Protein%2C+CF/pmc10753853-301-19-22
    Average 93 stars, based on 4 article reviews
    histagged cd11b - by Bioz Stars, 2026-09
    93/100 stars

    Images

    1) Product Images from "Toll-like receptor 4 and CD11b expressed on microglia coordinate eradication of Candida albicans cerebral mycosis"

    Article Title: Toll-like receptor 4 and CD11b expressed on microglia coordinate eradication of Candida albicans cerebral mycosis

    Journal: Cell reports

    doi: 10.1016/j.celrep.2023.113240

    (A) WT or CD11b −/− mice were challenged i.v. with 25,000 viable cells of WT or ece1 Δ/Δ C. albicans , and cerebral clearance was assessed over 20 days. (B–D) Schematic diagram of fungistasis assay (B) and aggregate data of candidalysin-activated BV-2 cells challenged with either WT or ece1 Δ/Δ C. albicans with and without anti-CD11b or NIF blockade of CD11b (C and D). (E and F) Fungistasis assay of candidalysin-activated (E) primary microglia or (F) primary astrocytes from WT mice with WT C. albicans with and without anti-CD11b or NIF blockade of CD11b. (G) Pull-down assays using CD11-expressing CHO cells. CHO-CD11a/b/c cell lysates (prey) were incubated with biotinylated candidalysin (Bio-Clys; bait) or biotinylated serine (Bio-serine; control), and the mixtures were loaded onto prewashed streptavidin beads. Bait-prey complexes were eluted from the beads and loaded onto SDS-PAGE gels to detect CD11a, CD11b, and CD11c via western blotting. (H–J) Schematic diagrams and aggregate data depicting in vitro assays in which the dose-dependent binding of plate-bound (H) CD11b extracellular domain or (I) candidalysin/SC peptide to the other reagent or (J) CD11b or CD11c extracellular domains binding to plate-bound candidalysin were determined colorimetrically (OD, optical density). (K) Flow cytometric analysis of splenocytes from WT or CD11b −/− mice incubated with AF647-conjugated CL (10 μM). n = 4, mean ± SEM, *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001 using one-way ANOVA followed by Tukey’s test for multiple comparison (A–F), two tailed Student’s t test (H–J), or two-way ANOVA (K). Data are representative of two or three independent experiments.
    Figure Legend Snippet: (A) WT or CD11b −/− mice were challenged i.v. with 25,000 viable cells of WT or ece1 Δ/Δ C. albicans , and cerebral clearance was assessed over 20 days. (B–D) Schematic diagram of fungistasis assay (B) and aggregate data of candidalysin-activated BV-2 cells challenged with either WT or ece1 Δ/Δ C. albicans with and without anti-CD11b or NIF blockade of CD11b (C and D). (E and F) Fungistasis assay of candidalysin-activated (E) primary microglia or (F) primary astrocytes from WT mice with WT C. albicans with and without anti-CD11b or NIF blockade of CD11b. (G) Pull-down assays using CD11-expressing CHO cells. CHO-CD11a/b/c cell lysates (prey) were incubated with biotinylated candidalysin (Bio-Clys; bait) or biotinylated serine (Bio-serine; control), and the mixtures were loaded onto prewashed streptavidin beads. Bait-prey complexes were eluted from the beads and loaded onto SDS-PAGE gels to detect CD11a, CD11b, and CD11c via western blotting. (H–J) Schematic diagrams and aggregate data depicting in vitro assays in which the dose-dependent binding of plate-bound (H) CD11b extracellular domain or (I) candidalysin/SC peptide to the other reagent or (J) CD11b or CD11c extracellular domains binding to plate-bound candidalysin were determined colorimetrically (OD, optical density). (K) Flow cytometric analysis of splenocytes from WT or CD11b −/− mice incubated with AF647-conjugated CL (10 μM). n = 4, mean ± SEM, *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001 using one-way ANOVA followed by Tukey’s test for multiple comparison (A–F), two tailed Student’s t test (H–J), or two-way ANOVA (K). Data are representative of two or three independent experiments.

    Techniques Used: Expressing, Incubation, Control, SDS Page, Western Blot, In Vitro, Binding Assay, Comparison, Two Tailed Test


    Figure Legend Snippet:

    Techniques Used: Recombinant, Control, Lysis, Magnetic Beads, Software, Enzyme-linked Immunosorbent Assay

    Related Articles

    Control:

    Article Title: Toll-like receptor 4 and CD11b expressed on microglia coordinate eradication of Candida albicans cerebral mycosis.
    Article Snippet: .. 96-well plates (9018, Corning, Kennebuck, ME) were coated with 5 mg/mL candidalysin or scrambled peptide control in carbonate buffer (pH = 9.0) overnight at 4 C. Plates were blocked with i-Block (2%) for 2 h at 37 C and incubated with a 2/3 serial dilution of Histagged CD11b (7959-AM, R&D systems, Minneapolis, MN) or CD11c (7987-AX, R&D systems, Minneapolis, MN), starting at 50nM for 2 h at 37 C. After washing, plates were incubated with biotinylated anti-His antibody (5 mg/mL, BAM050, R&D systems, Minneapolis, MN) for 2 h at 37 C followed by SAv-HRP (1:250, 51–9002813, BD Biosciences, San Jose, CA). .. Plates were further developed using TMB substrate solution (N301, ThermoFisher scientific, WalthamMA) and detected at 450 nm.

    Incubation:

    Article Title: Toll-like receptor 4 and CD11b expressed on microglia coordinate eradication of Candida albicans cerebral mycosis.
    Article Snippet: .. 96-well plates (9018, Corning, Kennebuck, ME) were coated with 5 mg/mL candidalysin or scrambled peptide control in carbonate buffer (pH = 9.0) overnight at 4 C. Plates were blocked with i-Block (2%) for 2 h at 37 C and incubated with a 2/3 serial dilution of Histagged CD11b (7959-AM, R&D systems, Minneapolis, MN) or CD11c (7987-AX, R&D systems, Minneapolis, MN), starting at 50nM for 2 h at 37 C. After washing, plates were incubated with biotinylated anti-His antibody (5 mg/mL, BAM050, R&D systems, Minneapolis, MN) for 2 h at 37 C followed by SAv-HRP (1:250, 51–9002813, BD Biosciences, San Jose, CA). .. Plates were further developed using TMB substrate solution (N301, ThermoFisher scientific, WalthamMA) and detected at 450 nm.

    Article Title: Toll-like receptor 4 and CD11b expressed on microglia coordinate eradication of Candida albicans cerebral mycosis
    Article Snippet: 96-well plates (9018, Corning, Kennebuck, ME) were coated with 5 μg/mL candidalysin or scrambled peptide control in carbonate buffer (pH = 9.0) overnight at 4°C. .. Plates were blocked with i-Block (2%) for 2 h at 37°C and incubated with a 2/3 serial dilution of Histagged CD11b (7959-AM, R&D systems, Minneapolis, MN) or CD11c (7987-AX, R&D systems, Minneapolis, MN), starting at 50nM for 2 h at 37°C. .. After washing, plates were incubated with biotinylated anti-His antibody (5 μg/mL, BAM050, R&D systems, Minneapolis, MN) for 2 h at 37C followed by SAv-HRP (1:250, 51–9002813, BD Biosciences, San Jose, CA).

    Serial Dilution:

    Article Title: Toll-like receptor 4 and CD11b expressed on microglia coordinate eradication of Candida albicans cerebral mycosis.
    Article Snippet: .. 96-well plates (9018, Corning, Kennebuck, ME) were coated with 5 mg/mL candidalysin or scrambled peptide control in carbonate buffer (pH = 9.0) overnight at 4 C. Plates were blocked with i-Block (2%) for 2 h at 37 C and incubated with a 2/3 serial dilution of Histagged CD11b (7959-AM, R&D systems, Minneapolis, MN) or CD11c (7987-AX, R&D systems, Minneapolis, MN), starting at 50nM for 2 h at 37 C. After washing, plates were incubated with biotinylated anti-His antibody (5 mg/mL, BAM050, R&D systems, Minneapolis, MN) for 2 h at 37 C followed by SAv-HRP (1:250, 51–9002813, BD Biosciences, San Jose, CA). .. Plates were further developed using TMB substrate solution (N301, ThermoFisher scientific, WalthamMA) and detected at 450 nm.

    Article Title: Toll-like receptor 4 and CD11b expressed on microglia coordinate eradication of Candida albicans cerebral mycosis
    Article Snippet: 96-well plates (9018, Corning, Kennebuck, ME) were coated with 5 μg/mL candidalysin or scrambled peptide control in carbonate buffer (pH = 9.0) overnight at 4°C. .. Plates were blocked with i-Block (2%) for 2 h at 37°C and incubated with a 2/3 serial dilution of Histagged CD11b (7959-AM, R&D systems, Minneapolis, MN) or CD11c (7987-AX, R&D systems, Minneapolis, MN), starting at 50nM for 2 h at 37°C. .. After washing, plates were incubated with biotinylated anti-His antibody (5 μg/mL, BAM050, R&D systems, Minneapolis, MN) for 2 h at 37C followed by SAv-HRP (1:250, 51–9002813, BD Biosciences, San Jose, CA).



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    (A) WT or <t>CD11b</t> −/− mice were challenged i.v. with 25,000 viable cells of WT or ece1 Δ/Δ C. albicans , and cerebral clearance was assessed over 20 days. (B–D) Schematic diagram of fungistasis assay (B) and aggregate data of candidalysin-activated BV-2 cells challenged with either WT or ece1 Δ/Δ C. albicans with and without anti-CD11b or NIF blockade of CD11b (C and D). (E and F) Fungistasis assay of candidalysin-activated (E) primary microglia or (F) primary astrocytes from WT mice with WT C. albicans with and without anti-CD11b or NIF blockade of CD11b. (G) Pull-down assays using CD11-expressing CHO cells. CHO-CD11a/b/c cell lysates (prey) were incubated with biotinylated candidalysin (Bio-Clys; bait) or biotinylated serine (Bio-serine; control), and the mixtures were loaded onto prewashed streptavidin beads. Bait-prey complexes were eluted from the beads and loaded onto SDS-PAGE gels to detect CD11a, CD11b, and CD11c via western blotting. (H–J) Schematic diagrams and aggregate data depicting in vitro assays in which the dose-dependent binding of plate-bound (H) CD11b extracellular domain or (I) candidalysin/SC peptide to the other reagent or (J) CD11b or CD11c extracellular domains binding to plate-bound candidalysin were determined colorimetrically (OD, optical density). (K) Flow cytometric analysis of splenocytes from WT or CD11b −/− mice incubated with AF647-conjugated CL (10 μM). n = 4, mean ± SEM, *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001 using one-way ANOVA followed by Tukey’s test for multiple comparison (A–F), two tailed Student’s t test (H–J), or two-way ANOVA (K). Data are representative of two or three independent experiments.
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    (A) WT or <t>CD11b</t> −/− mice were challenged i.v. with 25,000 viable cells of WT or ece1 Δ/Δ C. albicans , and cerebral clearance was assessed over 20 days. (B–D) Schematic diagram of fungistasis assay (B) and aggregate data of candidalysin-activated BV-2 cells challenged with either WT or ece1 Δ/Δ C. albicans with and without anti-CD11b or NIF blockade of CD11b (C and D). (E and F) Fungistasis assay of candidalysin-activated (E) primary microglia or (F) primary astrocytes from WT mice with WT C. albicans with and without anti-CD11b or NIF blockade of CD11b. (G) Pull-down assays using CD11-expressing CHO cells. CHO-CD11a/b/c cell lysates (prey) were incubated with biotinylated candidalysin (Bio-Clys; bait) or biotinylated serine (Bio-serine; control), and the mixtures were loaded onto prewashed streptavidin beads. Bait-prey complexes were eluted from the beads and loaded onto SDS-PAGE gels to detect CD11a, CD11b, and CD11c via western blotting. (H–J) Schematic diagrams and aggregate data depicting in vitro assays in which the dose-dependent binding of plate-bound (H) CD11b extracellular domain or (I) candidalysin/SC peptide to the other reagent or (J) CD11b or CD11c extracellular domains binding to plate-bound candidalysin were determined colorimetrically (OD, optical density). (K) Flow cytometric analysis of splenocytes from WT or CD11b −/− mice incubated with AF647-conjugated CL (10 μM). n = 4, mean ± SEM, *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001 using one-way ANOVA followed by Tukey’s test for multiple comparison (A–F), two tailed Student’s t test (H–J), or two-way ANOVA (K). Data are representative of two or three independent experiments.
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    (A) WT or CD11b −/− mice were challenged i.v. with 25,000 viable cells of WT or ece1 Δ/Δ C. albicans , and cerebral clearance was assessed over 20 days. (B–D) Schematic diagram of fungistasis assay (B) and aggregate data of candidalysin-activated BV-2 cells challenged with either WT or ece1 Δ/Δ C. albicans with and without anti-CD11b or NIF blockade of CD11b (C and D). (E and F) Fungistasis assay of candidalysin-activated (E) primary microglia or (F) primary astrocytes from WT mice with WT C. albicans with and without anti-CD11b or NIF blockade of CD11b. (G) Pull-down assays using CD11-expressing CHO cells. CHO-CD11a/b/c cell lysates (prey) were incubated with biotinylated candidalysin (Bio-Clys; bait) or biotinylated serine (Bio-serine; control), and the mixtures were loaded onto prewashed streptavidin beads. Bait-prey complexes were eluted from the beads and loaded onto SDS-PAGE gels to detect CD11a, CD11b, and CD11c via western blotting. (H–J) Schematic diagrams and aggregate data depicting in vitro assays in which the dose-dependent binding of plate-bound (H) CD11b extracellular domain or (I) candidalysin/SC peptide to the other reagent or (J) CD11b or CD11c extracellular domains binding to plate-bound candidalysin were determined colorimetrically (OD, optical density). (K) Flow cytometric analysis of splenocytes from WT or CD11b −/− mice incubated with AF647-conjugated CL (10 μM). n = 4, mean ± SEM, *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001 using one-way ANOVA followed by Tukey’s test for multiple comparison (A–F), two tailed Student’s t test (H–J), or two-way ANOVA (K). Data are representative of two or three independent experiments.

    Journal: Cell reports

    Article Title: Toll-like receptor 4 and CD11b expressed on microglia coordinate eradication of Candida albicans cerebral mycosis

    doi: 10.1016/j.celrep.2023.113240

    Figure Lengend Snippet: (A) WT or CD11b −/− mice were challenged i.v. with 25,000 viable cells of WT or ece1 Δ/Δ C. albicans , and cerebral clearance was assessed over 20 days. (B–D) Schematic diagram of fungistasis assay (B) and aggregate data of candidalysin-activated BV-2 cells challenged with either WT or ece1 Δ/Δ C. albicans with and without anti-CD11b or NIF blockade of CD11b (C and D). (E and F) Fungistasis assay of candidalysin-activated (E) primary microglia or (F) primary astrocytes from WT mice with WT C. albicans with and without anti-CD11b or NIF blockade of CD11b. (G) Pull-down assays using CD11-expressing CHO cells. CHO-CD11a/b/c cell lysates (prey) were incubated with biotinylated candidalysin (Bio-Clys; bait) or biotinylated serine (Bio-serine; control), and the mixtures were loaded onto prewashed streptavidin beads. Bait-prey complexes were eluted from the beads and loaded onto SDS-PAGE gels to detect CD11a, CD11b, and CD11c via western blotting. (H–J) Schematic diagrams and aggregate data depicting in vitro assays in which the dose-dependent binding of plate-bound (H) CD11b extracellular domain or (I) candidalysin/SC peptide to the other reagent or (J) CD11b or CD11c extracellular domains binding to plate-bound candidalysin were determined colorimetrically (OD, optical density). (K) Flow cytometric analysis of splenocytes from WT or CD11b −/− mice incubated with AF647-conjugated CL (10 μM). n = 4, mean ± SEM, *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001 using one-way ANOVA followed by Tukey’s test for multiple comparison (A–F), two tailed Student’s t test (H–J), or two-way ANOVA (K). Data are representative of two or three independent experiments.

    Article Snippet: Plates were blocked with i-Block (2%) for 2 h at 37°C and incubated with a 2/3 serial dilution of Histagged CD11b (7959-AM, R&D systems, Minneapolis, MN) or CD11c (7987-AX, R&D systems, Minneapolis, MN), starting at 50nM for 2 h at 37°C.

    Techniques: Expressing, Incubation, Control, SDS Page, Western Blot, In Vitro, Binding Assay, Comparison, Two Tailed Test

    Journal: Cell reports

    Article Title: Toll-like receptor 4 and CD11b expressed on microglia coordinate eradication of Candida albicans cerebral mycosis

    doi: 10.1016/j.celrep.2023.113240

    Figure Lengend Snippet:

    Article Snippet: Plates were blocked with i-Block (2%) for 2 h at 37°C and incubated with a 2/3 serial dilution of Histagged CD11b (7959-AM, R&D systems, Minneapolis, MN) or CD11c (7987-AX, R&D systems, Minneapolis, MN), starting at 50nM for 2 h at 37°C.

    Techniques: Recombinant, Control, Lysis, Magnetic Beads, Software, Enzyme-linked Immunosorbent Assay